The air inlet of the purification workbench is on the back or under the front, and there is an ordinary foam plastic sheet or non-woven fabric in the metal mesh cover to block large particles of dust. It should be checked, removed and washed frequently. If the foam is found to be aging, it should be replaced in time. Except for the air inlet, if there are leaking holes, they should be sealed tightly, such as sticking tape, stuffing cotton, sticking glue paper, etc. There is a super filter in the metal mesh cover on the front of the workbench. The super filter can also be replaced. If it is used for a long time, the dust particles are clogged, the wind speed is reduced, and the aseptic operation cannot be guaranteed, it can be replaced with a new one.
The sterile room should be sprayed with 70% alcohol or 0.5% phenol to reduce dust and disinfect regularly. Use 2% Xinjieer to wipe the countertops and utensils. 70% alcohol is also possible. Use formalin? 40% formaldehyde plus a small amount of permanganic acid. Potassium is regularly sealed for fumigation, combined with ultraviolet sterilization lamps? Turn on for more than 15 minutes each time and other disinfection and sterilization methods, so that the sterile room can always maintain a high degree of sterility. The inside of the inoculation box should also be equipped with an ultraviolet light. Turn on the light for more than 15 minutes before using it for sterilization. However, there are still bacteria in the place where it is not exposed. When the UV lamp is turned on for a long time, it can stimulate the oxygen molecules in the air to associate to ozone molecules. This gas component has a strong sterilization effect and can sterilize the corners that are not directly illuminated by the ultraviolet rays. Since ozone is a health hazard, you should turn off the ultraviolet light before entering the operation, and you can enter it more than ten minutes after turning off.
The UV lamp can also be hoisted on the clean bench, but it should be installed outside the lampshade, and staggered and arranged in parallel, so as not to hinder the lighting during work. It is useless if the ultraviolet lamp is installed in the illuminating lampshade? glass plate, because ultraviolet rays cannot penetrate glass, and its lamp tube is made of quartz glass, not silicate glass.
The inoculation room strives to be concise, and all items that are not directly related to the work of the room cannot be put in to facilitate the maintenance of sterility. The air in the inoculation room should be absolutely isolated from the outside air, and the reserved air vents should be as tight as possible. The air vents can be equipped with upper and lower louvers. The area of the louvers should be slightly larger and need to be covered with 4 layers of gauze for simple dust filtering. After a day's work, the window can be opened to fully ventilate, and then sealed. In short, it must be clean, dust-free and sterile, and fresh air, suitable for work. The gauze covering the vent window should be changed and washed frequently. However, the above-mentioned measures are only ideal design schemes, which are often not easy to achieve comprehensively. In fact, as long as strict aseptic operation procedures, in the room with open doors and windows, and the protection of a clean bench, the pollution rate of inoculation can still be controlled and can be tolerated in production. Level.
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